Isolation and characterization of synovial mesenchymal stem cell derived from hip joints. A comparative analysis with a matched control knee group
Akihisa Hatakeyama1, Soshi Uchida2, Hajime Utsunomiya2, Manabu Tsukamoto1, Hirotaka Nakashima１, Eiichiro Nakamura1, Cecilia Pascual-Garrido4, Ichiro Sekiya3, Akinori Sakai1
が Stem Cell International
この研究は 股関節と膝関節を 同日に手術を行う患者さんから 同意を得て、 不要な滑膜組織を採取して 細胞を培養し、膝と股関節の間葉系幹細胞としての ポテンシャルを調べたものであります。
膝の滑膜ほうが 脂肪成分が多く、股関節の滑膜は 繊維成分が多い。
増殖能や脂肪分化能は 膝の滑膜間葉系幹細胞の方が 股関節の滑膜間葉系幹細胞より、すぐれている
Purpose: To determine the characteristics of MSCs from hip synovial tissue and compare them to MSCs from knee synovial tissue.
Methods: Synovial fluid and tissues were obtained from both the knee and the hip joints in 8 patients who underwent both hip and knee arthroscopies on the same day. . MSCs were isolated from the knee and hip synovial samples. Number of cells, colony forming units, viability and differentiation capacities of adipose, cartilage and bone were compared between both groups. In addition, RT-PCR was used to assess the differentiation capacity of adipose, cartilage and bone tissue from both samples.
Results: The number of cells per unit weight at passage 0 of synovium from the knee was significantly higher than that from the hip (p < 0.05). There was no significant difference in colony-forming units between synovium from the hip and the knee. While it was possible to observe the growth of colonies in all the knee synovial fluid samples, it was completely impossible to culture cells from any of the hip synovial fluid samples. In adipogenesis experiments, the frequency of Oil red O-positive colonies and the gene expression of adipsin were significantly higher in knee than that in hip. In osteogenesis experiments, there was no significant difference in the number of von Kossa and alkaline phosphatase positive colonies between the knee and the hip. Quantitative RT-PCR demonstrated that the expression of COL1A1 and ALPP was significantly less in the knee synovium than in the hip synovium (p < 0.05). The expression of RUNX2 and BGLAP was significantly higher in the knee than in the hip (p < 0.05). In chondrogenesis experiments, the expression of COL2A1 was higher in the knee than in the hip (p < 0.05).
Conclusions: MSCs obtained from hip synovial tissue have self-renewal and multi-lineage differentiation potentials. However, in matched donors, adipogenesis and osteogenesis potentials of MSCs from the knees are superior to those from the hips. Knee synovium may be a better source of MSC for potential use in hip diseases compared to hip synovium.