ݡʰS. UΥ֥ Sports Physician S.U Blog

Dr. Soshi Uchida of Wakamatsu hospital for sports injury is an orthopedic surgeon dedicated to research and treatment of hip, shoulder, knee and elbow sports injuries We will do our best for the treatment for the patients with smile and special skills. 벼ѤΥƥ˥åȤơǾǴԤŤƤɥ֤륹ݡİؤξͥǤ 22 ǯ֤J꡼Υɥٻ̻ˢʬǯJ꡼ꤷ̶彣ˤηиȡȤνƻʣʡˤȥ饰ӡ򤷤ƤƤꡢݡŤƤޤλ̣ϡдǿǻޤ 򤷤ͦͿ뤳ȤåȡȤƤޤʥȥؤذαؤǯ֤ƤꡢѸä볰ͤοǻ⤷ƤޤnSports Physician SU Sports Medicine Blog

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Dr Soshi Uchida MD, PhD has been practicing his passion, orthopaedic surgery, for the last decade. The principles of sports medicine emphasize a comprehensive team approach in the conservative treatment of injuries with the goal of helping the patient return to a healthy state, and focuses on the prevention of new or recurrent injuries. When surgery is necessary, Dr. Uchida's specialty in arthroscopic techniques, especially hip arthroscopy, emphasizes less invasive surgery for a potentially easier and quicker recovery. These principles, initially developed for the competitive athlete, apply to all injuries whether they occur in the recreational environment, work place, or at home. He is a well-known figure in hip arthroscopic surgery, lecturing, travelling and teaching widely. In addition he has contributed extensively to the design and development of special instruments for arthroscopic surgery of the hip joint. Many patients living in Fukuoka, Nagasaki, Oita, Osaka, Kobe, Kyoto, Tokyo, Yokohama present to our hospital for their hip problem.

American Journal of Sports Medicine ʸ


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Am J Sports Med. 2018 Sep 14:363546518794664. doi: 10.1177/0363546518794664. [Epub ahead of print]
Synovial Mesenchymal Stem Cells Derived From the Cotyloid Fossa Synovium Have Higher Self-renewal and Differentiation Potential Than Those From the Paralabral Synovium in the Hip Joint.
Murata Y1, Uchida S2, Utsunomiya H2, Hatakeyama A2, Nakashima H1, Chang A3, Sekiya I4, Sakai A1.
Author information
Abstract
BACKGROUND:
Several studies have shown the relationship between poorer clinical outcomes of arthroscopic femoroacetabular impingement syndrome surgery and focal chondral defects or global chondromalacia/osteoarthritis. Although recent studies described good outcomes after the conjunctive application of synovial mesenchymal stem cells (MSCs), none demonstrated the application of synovial MSCs for cartilaginous hip injuries.

PURPOSE:
To compare the characteristics of MSCs derived from the paralabral synovium and the cotyloid fossa synovium and determine which is the better source.

STUDY DESIGN:
Controlled laboratory study.

METHODS:
Synovium was harvested from 2 locations of the hip-paralabral and cotyloid fossa-from 18 donors. The number of cells, colony-forming units, viability, and differentiation capacities of adipose, bone, and cartilage were collected and compared between groups. In addition, real-time polymerase chain reaction was used to assess the differentiation capacity of adipose, bone, and cartilage tissue from both samples.

RESULTS:
The number of colonies and yield obtained at passage 0 of synovium from the cotyloid fossa was significantly higher than that of the paralabral synovium ( P < .01). In adipogenesis experiments, the frequency of detecting oil red O-positive colonies was significantly higher in the cotyloid fossa than in the paralabral synovium ( P < .05). In osteogenesis experiments, the frequency of von Kossa and alkaline phosphatase positive colonies was higher in the cotyloid fossa synovium than in the paralabral synovium ( P < .05). In chondrogenic experiments, the chondrogenic pellet culture and the gene expressions of COL2a1 and SOX9 were higher in the cotyloid fossa synovium than in the paralabral synovium ( P < .05).

CONCLUSION:
MSCs from the cotyloid fossa synovium have higher proliferation and differentiation potential than do those from the paralabral synovium and are therefore a better source.

CLINICAL RELEVANCE:
Synovial cells from the cotyloid fossa synovium of patients with femoroacetabular impingement syndrome are more robust in vitro, suggesting that MSCs from this source may be strongly considered for stem cell therapy.

KEYWORDS:
cotyloid fossa synovium; femoroacetabular impingement syndrome (FAIS); regeneration therapy of hip joint; synovial mesenchymal stem cells (MSCs)





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Isolation and characterization of synovial mesenchymal stem cell derived from hip joints. A comparative analysis with a matched control knee group

Akihisa Hatakeyama1, Soshi Uchida2, Hajime Utsunomiya2, Manabu Tsukamoto1, Hirotaka Nakashima, Eiichiro Nakamura1, Cecilia Pascual-Garrido4, Ichiro Sekiya3, Akinori Sakai1


Stem Cell International


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Abstract
Purpose: To determine the characteristics of MSCs from hip synovial tissue and compare them to MSCs from knee synovial tissue.
Methods: Synovial fluid and tissues were obtained from both the knee and the hip joints in 8 patients who underwent both hip and knee arthroscopies on the same day. . MSCs were isolated from the knee and hip synovial samples. Number of cells, colony forming units, viability and differentiation capacities of adipose, cartilage and bone were compared between both groups. In addition, RT-PCR was used to assess the differentiation capacity of adipose, cartilage and bone tissue from both samples.
Results: The number of cells per unit weight at passage 0 of synovium from the knee was significantly higher than that from the hip (p < 0.05). There was no significant difference in colony-forming units between synovium from the hip and the knee. While it was possible to observe the growth of colonies in all the knee synovial fluid samples, it was completely impossible to culture cells from any of the hip synovial fluid samples. In adipogenesis experiments, the frequency of Oil red O-positive colonies and the gene expression of adipsin were significantly higher in knee than that in hip. In osteogenesis experiments, there was no significant difference in the number of von Kossa and alkaline phosphatase positive colonies between the knee and the hip. Quantitative RT-PCR demonstrated that the expression of COL1A1 and ALPP was significantly less in the knee synovium than in the hip synovium (p < 0.05). The expression of RUNX2 and BGLAP was significantly higher in the knee than in the hip (p < 0.05). In chondrogenesis experiments, the expression of COL2A1 was higher in the knee than in the hip (p < 0.05).
Conclusions: MSCs obtained from hip synovial tissue have self-renewal and multi-lineage differentiation potentials. However, in matched donors, adipogenesis and osteogenesis potentials of MSCs from the knees are superior to those from the hips. Knee synovium may be a better source of MSC for potential use in hip diseases compared to hip synovium.


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Dr. S.U

Dr Soshi Uchida MD, PhD has been practicing his passion, orthopaedic surgery, for the last two decade. The principles of sports medicine emphasize a comprehensive team approach in the conservative treatment of injuries with the goal of helping the patient return to a healthy state, and focuses on the prevention of new or recurrent injuries. When surgery is necessary, Dr. Uchida's specialty in arthroscopic techniques emphasizes less invasive surgery for a potentially easier and quicker recovery.
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